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KMID : 0381120110330050541
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Genes and Genomics
2011 Volume.33 No. 5 p.541 ~ p.541
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Molecular structure and developmental expression of zebrafish atp2a genes
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Lai Yen-Yu
Pai Chiung-Wen
Tsai I.-Ting
Chou Chi-Yuan
Tsai Chia-Ti
Chen Yau-Hung
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Abstract
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We isolated two atp2a genes, atp2a1 and atp2a2a, from embryonic zebrafish. Amino acid sequences deduced from zebrafish atp2a genes are aligned with orthologue proteins from other species, the results showed that they share high percentage of identities (82%?94%) and acidic pIs (5.03?5.33). Whole mount in situ hybridization experiments showed that atp2a1 and atp2a2a are maternal inherited genes which can be detected at 1-cell stage embryos and express in the entire animal pole from 6 hours post-fertilization (hpf) to 12 hpf. At the later stages (48?96 hpf), expression of atp2a1 was restricted in head and trunk muscles as well as in some neurons. In contrast to the strongly expression of atp2a1 in head muscle, expression of atp2a2a was detected in head muscle in a fainter manner. In addition, transcripts of atp2a2a were observed in the developing heart during early cardiogenesis. The present studies not only help us to comparatively analyze atp2a genes across species, but also provide useful information about expressions during early embryogenesis that will help in further investigations of functional studies of Atp2a in the future.
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KEYWORD
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Calcium, Cardiogenesis, Muscle, SERCA, Zebrafish
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